In this section
Below are a list of frequently asked questions. Please don't hesistate to get in contact if you have any further questions or need clarifications.
Visit our support page for tutorials and contact information.
How long will the system run for?
As long as is needed. Our systems run and record 24 hours a day, 7 days a week.
How many rodents can be tested in one cage?
We have tested with five, but three is optimal to achieve highly accurate identity retention and tracking.
Is the system different for mice and rats?
Yes. Mice and rats obviously differ in size, as do their cages, so our systems are calibrated accordingly. The RFID chip is smaller for mice, as is the base plate. Because the chip is smaller, temperature is not currently recorded within mice studies, although work to amend this is in progress.
Do I need to fund any of my own equipment?
Installation, support, and the entire system are all included. You only need to provide your own standard cages and rodents.
What about different enrichment, such as "huts", and what that will do to activity? What about different bedding?
Bedding comes in various forms, but very thick bedding can lead to poorer data. Enrichment is no problem; red plastic tubes are very easy to integrate as the HCA's infra-red camera can see right through them. If you use items like toilet roll tubes the cameras will not pick up activity inside them, but the positional data collection still works very well and identity is still retained.
How quickly can one get data from the system to be analysed?
The first experimental data can be accessed after 15 minutes. Each cage has an individual local computer that gathers the raw data: the video feed and the feed from the baseplate. This data is stored safely locally and gathered by a server unit every few minutes. If you have outside access to your server, you can access this raw data remotely. Within minutes of the trial starting, you start to receive data.
All video is stored by default, and you have full control over the storage and retention of the data. As soon as the data reaches the server the analysis begins, in order to overlay the behavioural analytics onto the data. It is also possible to conduct analysis during the experiment – the experimental data can be exported into a statistical package like Microsoft Excel for further study.
What, if any, are the size restrictions for the rodents? Is there a suggested body weight range to ensure proper tracking?
We have not come across any animals that are too large or small yet. Very young pups may be too small for the RFID tags.
How do you analyse the "cage" factor in pharmacology studies when animals are housed in collective cages?
In collective cages you can run all animals under the same treatment regime or put them in mixed groups. The choice depends on the study design and what other tests are planned for the animals. With either variation, individual identity retention and tracking means you can automatically measure interactions between cage mates during the study. You will also have the videos if specific dominant traits need to be confirmed.
Can you talk more about the automated scoring algorithms? What tracking information is going into the classifiers? Can the user train new classifiers for behaviours?
Each algorithm is different. For any new behaviour we typically collect a set of ground truth annotated examples in the system in collaboration with an expert user in the domain. We then review a small selection, propose draft solutions, and implement a test version that we refine on the larger dataset (retaining samples for validation testing).
Once the user and ourselves are satisfied that the algorithm is useful we integrate it into the suite that is run on the analysis server. Once added, revisions can be rolled out as it is improved. At present users cannot train their own classifiers within the system and will need to work with us.
Are there specific brands or types of IVC units it is designed for? Any restrictions?
Yes, we have working systems using Techniplast Blue Line and Green Line and Allentown Type 2 cages. Others are available upon request.
How are the subcutaneous sensors injected? What is the optimal anatomical region to position the RFID coil in the mice and rat?
They are injected using standard injectors supplied by the vendors. The preferred position is in the groin and there are SOPs available for the procedure.
How do you deal with animals being hidden behind cage mates, or bedding/nesting material?
The baseplate and RFID do the majority of that work, even when the animals are hidden you can still identify who is who, their location, and how mobile they are. The camera experiences rare occlusion events, but these are minor in the context of the entire multi-day data set.
What are the computer and network requirements (infrastructure) on the front and back end to adequately drive the analysis software and collect data?
We supply the front-end software with each system. The back end requires a server, but we have designed such that low specification workstations/servers can be used. Most of our customers use sub-$2000 servers for four to eight cages. The systems need network access (typically DHCP) but this can be within a confined intranet. 100BaseT or gigabit Ethernet is ideal. The systems can be accessed remotely, but this needs to be handled by your local network support team.
Is the behavioural scoring being performed via video or the base plate?
It’s a combination of both, depending on the behaviour.
Can the data be proofed during long-term experiments?
The server constantly pulls data off the capture notes during long term studies. This can easily be configured to be redundant/backed up as required, even during the study.
What are the 3Rs benefits?
Refinement: The behavioural data collected is entirely non-invasive and based on observing group behaviour in the home cage.
Reduction: The quantity of longitudinal data gathered provides additional statistical power, meaning fewer animals are required.
Replacement: Use data stored on your computer from previous experiments to reanalyse using different parameters. For more information, see here.
What else can the system be used for beyond the examples given?
The system will automatically detect abnormal motor events. Instead of examining hours of video footage, you can simply manually assess the abnormal behaviour and verify the event; a convulsion, for example. ActualHCA also offers exciting possibilities for defining aggression better and to time mating properly, which in turn offers the opportunity to study fertility much more closely.
As well as this, the opportunity to study the social interaction of rodents within their true home cage is valuable. The system is designed to ensure that all the raw data is securely stored, for as long as you need or want it. If you want to apply new algorithms to existing footage instead of repeating an experiment, you can.
Can the system be used and controlled remotely?
There is the ability to export a live feed. If you have the appropriate network connections, you can see what's happening live or log into the server with a short delay.
The system requires very little configuration once it's up and running. Studies can use ActualHCA successfully for days, weeks, or months.
Once you have a rack installed, is it fixed or can the modules be taken in and out as needed?
The system is physically installed into the rack, but the cages can be easily swapped in and out for welfare checks. Your lab will run just as smoothly as it currently does, with no extra space needed and no requirement to adapt your laboratory practice. If this isn't suitable, a desktop version of ActualHCA is available.